Detection of arginase 1arg1 in hepg2 human cell line by flow cytometry. The urea cycle is a metabolic process in the body, which produces ammonia during breakdown. Tested in western blot wb, immunohistochemistry frozen ihc f and immunohistochemistry paraffin ihc p applications. Cuando una enzima llamada arginasa falta o trabaja a niveles anormales. Detection of arginase 1arg1 in hepa 16 mouse cell line by flow cytometry. In humans, this protein is encoded by the gene arg1. This antibody reacts with human, mouse, rat samples. An enzyme found primarily in the liver that catalyzes the hydrolysis of arginine to form urea and ornithine. Antiarginase1 antibodies are available from several suppliers. Arginase 2 antibody pa527987 thermo fisher scientific. Urea cycle is very important in eliminating nitrogen waste from the body.
Arginase activity assay kit mak112 technical bulletin. Arginase1 arg1 is a key enzyme of the urea cycle found in liver that catalyzes the conversion of larginine into lornithine and urea. Arginase activity assay kit catalog number mak112 technical bulletin product description arginase is a manganesecontaining enzyme that catalyzes the conversion of arginine to urea and ornithine. Downs from, the department of pathological chemistry, universitu nf toronto, toronto, canada received for publication, july 24, 1944 in the course of an attempt to develop a rapid method for the enzymatic determination of arginine it was observed that a quantity of. Arginase1 deficiency nord national organization for. Immunoprecipitation of arginase2 from k562 cell extracts.
Pdf arginase is an enzyme that metabolizes larginine to lornithine and urea. The protein may also be known as arginase, liver, arginase i, arginase, liver, and livertype arginase. Arginase1 deficiency is a rare inherited disorder characterized by complete or partial lack of the enzyme arginase in the liver and red blood cells. Etiology the main cause behind arginase deficiency disorder is the absence of a very crucial enzyme in the urea cycle.
A mutated arg1 gene may result in an arginase enzyme that is unstable, shorter than usual or the wrong shape, or may prevent the enzyme from being produced at all the shape of an enzyme affects its ability to control a chemical reaction. Arginase is one of six enzymes that play a role in the breakdown and removal of nitrogen from the body, a process known as the urea cycle. Arginase1 arg1 deficiency is a rare autosomal recessive disorder that affects the liverbased urea cycle, leading to impaired ureagenesis. If the arginase enzyme is misshapen or missing, it cannot. The lack of the arginase enzyme results in excessive. Western blot analysis was performed using arginase2 antibody. Smallmolecule arginase inhibitors are currently described as. Approximately 12 mutations have been identified in the arg1 gene. Two isoforms of arginase are present in most mammals, which differ in their tissue distribution and subcellular localization.
What are the causes of arginase deficiency disorder. Arg1 is a highly specific and sensitive marker of benign and hepatocellular carcinoma hcc which is now a key target for the differential diagnosis of hcc from metastatic tumors to the liver. Arginase definition of arginase by the free dictionary. Humanmouse arginase 1arg1 peconjugated antibody ic5868p. Argininemia, is an autosomal recessive urea cycle disorder where a deficiency of the enzyme arginase causes a buildup of arginine and ammonia in the blood. Ammonia, which is formed when proteins are broken down in the body, is toxic if levels become too high.
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